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After a 30-min stabilization incubation, PBS (without IBMX) and 100 M 3-isobutyl-1-methylxanthine (with IBMX) (Sigma Chemicals, St Louis Mo) was added and incubated for 60 min so that each rat served as its own control

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(88) in an 8-week randomized double-blind, placebo-controlled trial with 80 individuals experiencing episodic migraines, discovered that the combination of omega-3 PUFAs and nano-curcumin significantly reduced migraine attack frequency and serum levels of IL-1 in a synergistic manner, though the initial significant reduction in IL-1 gene expression did not remain significant after Bonferroni corrections