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The cells were then incubated with antibodies against arrestin3 or HA (1:1000 dilution) for 1 h at 20 C, followed by washing with PBS and incubation with the secondary antibodies (Alexa 555-conjugated antibodies at 1:500 dilution)

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vii , viii Conversely, studies in numerous species of animals have shown that boosting glutathione levels results in better health and increased longevity, and analyses in humans indicate that higher glutathione levels are associated with better health and increased longevity