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GTP turnover was then started by adding GTP (5 M) and GDP (10 M), and the amount of remaining GTP was assessed after 10 min incubation at 15 C using the GTPase-Glo assay (Promega)

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In vivo Assessment of Drug Interactions Empagliflozin pharmacokinetics were similar with and without coadministration of metformin, glimepiride, pioglitazone, sitagliptin, linagliptin, warfarin, verapamil, ramipril, and simvastatin in healthy volunteers and with or without coadministration of hydrochlorothiazide and torsemide in patients with type 2 diabetes mellitus (see Figure 1)