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In vitro assays with 3,4- cis -leucocyanidin (6) Standard reaction conditions for in vitro one-step and two-step biotransformations of 3,4- cis -leucocyanidin ( 6 ) were 100 mM MOPSO, pH 6.2, 20 mM sodium ascorbate, 1 mM -ketoglutaric acid, 0.4 mM ammonium iron(II) sulfate, 200 M 3,4- cis -leucocyanidin ( 6 ) and 20 M GSH