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Their method involved adding SRBCs to mouse spleen cells in Petri dishes and then charting the formation of plaques which signified the emergence of cells that secreted antibodies to the SRBCs (Mishell, Dutton)

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The positive cell of c-Fos activation was quantified by using the Fiji software (v 2.1.0/1.53c)

These conflicting results suggest that further mechanistic research is needed to investigate the influences of each SCFA or in combination on appetite control in order to precisely and systematically explore the relationship between SCFAs and appetite control