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The reconstitution process must be done gentlyno shaking!to avoid damaging the peptide chains

To explore the substrate-binding sites in both the dimeric and stacked assemblies, we superimposed the COP1 WD40 with Trib1 (homolog of Drosophila tribbles), which are adaptors for C/EBP degradation (PDB: 5IGQ) onto the individual COP1 WD40 domain in these two states of structures (Supplementary Fig

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