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Curcumin-induced upregulation of SIRT1 has been associated with beneficial effects on various diseases, including cardiac fibrosis, diabetes, and ischemia/reperfusion injury [141,142]

In another set of experiments, ASA treatments (5 or 10 mol/ml ASA 48 h) were performed in GSH-depleted cells (BSO treatment for 18 h) which were then treated with 10 mM NAC (dissolved in DMSO, then diluted with cell culture medium to the required concentration keeping the DMSO concentration not more than 0.1%) for 2 hr before the addition of ASA for 48 h to manipulate cellular GSH pools

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