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Multi-agency participation : Eleven federal agencies participate, including NIH, NSF, DoD, DOE, NASA, USDA, DHS, and others

Panossian A, Wikman G

The H-ESI source was operated in positive mode at spray voltage 3,500 or negative mode at spray voltage 2,500 with the following parameters: sheath gas 35 a.u., aux gas 7 a.u., sweep gas 0 a.u., ion transfer tube temperature 320 C, vaporizer temperature 275 C, mass range 70 to 1,000 m/z , full scan MS1 mass resolution of 120,000 FWHM, RF lens at 70% and standard AGC

A 2-ml sample volume was taken at regular intervals to maintain sink conditions and replaced with an equivalent volume of fresh buffer