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Selection with puromycin (1 g/mL) was performed in 10% FBS culture medium

Thus, it can be summarized that morphological alterations typical of eryptosis, including cell shrinkage and membrane microvesiculation, are governed by the elevation of intracellular calcium ion levels

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Alignment of amino acid sequences shows that TmGpx1 shares a high identity with Aspergillus fumigatus glutathione peroxidase Hyr1 (77%), Histoplasma capsulatum glutathione peroxidase (73%), Coccidioides immitis glutathione peroxidase Hyr1 (73%), and Paracoccidioides brasiliensis Pb18 glutathione peroxidase (67%)