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Sometimes the cake of lyophilized powder is quite dense and takes a few minutes to break down

To promote in vivo recombination in yeast, the genes were PCR-amplified to introduce specific overhangs of 4055 bp to the linearized vector using iProof high-fidelity polymerase (Bio-Rad, 1725301) following the manufacturers specifications

Consequently, a comprehensive and systematic understanding of the specific molecular regulatory mechanisms of mitochondrial quality control using novel technologies and methodologies is a pivotal objective of future research (Figure 4)

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