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Nevertheless, some drawbacks such as derivatization, cumbersome laboratory procedures, time consumption and applicable feasibility in vivo were not conducive to the development of a practical method

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Since the Lewis lung carcinoma (LLC) model is the only reproducible syngeneic model for NSCLC [15], we deemed it is appropriate for determining the effect of IGF1R deficiency on key components of the TME, not only using an experimental pulmonary metastasis model but also upon heterotopic syngeneic transplantation