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This step and the next step of Western blotting were necessary, because the proteins evaluated in the Kinex TM KAM 1.0 antibody microarrays were not denatured, which increases the opportunity for false positives and false negatives due to antibody cross-reactivity, protein-protein interactions, and blocked epitopes in protein complexes

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Under a 2009 law, the FDA gained authority to regulate certain parts of the tobacco industry, including nicotine in cigarettes, though it cannot remove the ingredient completely

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