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Briefly, 4-m sections of formalin-fixed and paraffin-embedded tissues were deparaffinized using Bond Dewax Solution (Leica Biosystems Newcastle Ltd.) and antigen retrieval was achieved by incubation with Bond Epitope Retrieval Solution 1 (Leica Biosystems Newcastle Ltd.) for 20 minutes at 98

Nature 2016;532:255-8

Although MYCN downregulation or overexpression are in the region of 50% or more, they are still within the natural range of variation observed in MNA and MYCN non-amplified cells, respectively ( Figure 4 To further measure cell death upon treatment with MitoQ, DPI and vincristine, we measured caspase 3/7 activation using Caspase-Glo 3/7 (Promega) ( Figure 4D )

Ivanova, V