glutathione reductase assay protocol Assays To Monitor Oxidative Stress picking an oxidative damage assay

doi: 10.1089/ars.2012.5052 29 HernndezJ

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A comprehensive analysis of GSH and GABA levels in multiple brain regions, including the primary motor cortex (SM1), thalamus (Thal), medial prefrontal cortex (mPFC), and supplementary motor area (SMA), was conducted using single-voxel HERMES MR spectroscopy at 3T

A 3 mL of reaction mixture was prepared with 0.1 mL of 13 mM L-methionine, 0.1 mL of 75 M p - nitroblue terazolium chloride (NBT), 0.1 mL of 100 M EDTA, 0.1 mL riboflavin (2 M) in a 1.5 mL of 50 mM potassium phosphate buffer pH 7.8, 50 L of the enzymatic extract and distilled water