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Since the glucose-lowering effects of GIP have been shown to be impaired in patients with T2D, interest has focused on developing antidiabetic therapies based upon the actions of GLP-1 and GIP, the response to which remains intact

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Briefly, 2 g of PGA (Sigma-Aldrich, 95%) were weighed into a 50 mL volumetric flask and supplemented with 15 mL of 0.02 N methanolic sulfuric acid, with the methanol consisting of either 5% 13 C-labeled methanol (Sigma Aldrich, 99%, 99 atom% 13 C) or unlabeled methanol (Sigma-Aldrich, 99.8%)

Fischer KE, Nagaraj G, Daniels RH, Li E, Cowles VE, Miller JL, Bunger MD, Desai TA