Discrepancies were observed between the qRT-PCR results and the RNA-seq data, likely due to different sampling times, tissue types, and variations in the FPKM (fragments per kilobase of transcript per million mapped reads) values [71]
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Furthermore, the chemical composition of the essential oil was characterized using gas chromatographymass spectrometry (GC-MS), and molecular docking analyses were performed to evaluate the binding affinity of its major constituents with the three key TMV proteins (replicase, movement, and coat)
