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Lysates were centrifuged at 14,000 rpm for 15 min, and protein concentration was determined using the BCA kit (Thermo Fisher, 23227)

Seek urgent advice if you experience symptoms of lactic acidosis (a rare but serious complication associated with metformin), including unusual muscle pain, difficulty breathing, severe nausea or vomiting, abdominal pain, feeling extremely weak or tired, or unusual sleepiness

WangRXiaoMZhangYHoCTWanXLiDet alRNA-sequencing analysis reveals L-theanine regulating transcriptional rhythm alteration in vascular smooth muscle cells induced by dexamethasone

This aligns with evidence that mitochondrial fission can proceed without changes in DRP1 expression, such as with deletion of PTEN-induced kinase 1 or protein kinase A activation, which affect DRP1 phosphorylation at Ser616 without altering protein levels [61, 62]