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At present, research on tobaccos response to chilling injury is largely restricted to laboratory-simulated environments, where low-temperature stress is induced by controlling temperature to investigate the physiological and molecular responses of tobacco ( We hypothesize that low-temperature stress in field conditions significantly reduces the physiological functions of tobacco by inhibiting photosynthesis, disrupting the antioxidant system, regulating the expression of related proteins, and altering metabolite accumulation, and its effects are further complicated by the interactions with environmental factors