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Common Cagrilintide Reconstitution Setups Vial Size 5 mg BAC Water 2.0 mL Concentration 2.5 mg/mL 0.6 mg Draw 0.24 mL / 24 units 1.2 mg Draw 0.48 mL / 48 units 2.4 mg Draw 0.96 mL / 96 units 4.5 mg Draw 1.80 mL / 180 units* Vial Size 5 mg BAC Water 3.0 mL Concentration 1.67 mg/mL 0.6 mg Draw 0.36 mL / 36 units 1.2 mg Draw 0.72 mL / 72 units 2.4 mg Draw 1.44 mL / 144 units* 4.5 mg Draw Exceeds vial Vial Size 10 mg BAC Water 2.0 mL Concentration 5.0 mg/mL 0.6 mg Draw 0.12 mL / 12 units 1.2 mg Draw 0.24 mL / 24 units 2.4 mg Draw 0.48 mL / 48 units 4.5 mg Draw 0.90 mL / 90 units Vial Size 10 mg BAC Water 3.0 mL Concentration 3.33 mg/mL 0.6 mg Draw 0.18 mL / 18 units 1.2 mg Draw 0.36 mL / 36 units 2.4 mg Draw 0.72 mL / 72 units 4.5 mg Draw 1.35 mL / 135 units* *Draws above 1.0 mL exceed standard 1 mL U-100 syringe capacity and may require a larger syringe or split injections

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Abbreviations BC: -Carotene DOX: Doxorubicin BCDOX: -Carotene and doxorubicin co-loaded liposomes CRC: Colorectal cancer EPR: Enhanced permeability and retention DLS: Dynamic light scattering TEM: Transmission electron microscopy FTIR: Fourier-transform infrared spectroscopy DSC: Differential scanning calorimetry PDI: Polydispersity index EE%: Entrapment efficiency percentage IC 50 : Half maximal inhibitory concentration ROS: Reactive oxygen species UVVis: Ultravioletvisible spectroscopy SRB: Sulforhodamine B (cytotoxicity assay) MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (viability assay, if used) PBS: Phosphate-buffered saline ANOVA: Analysis of variance CI: Combination index HPLC: High-performance liquid chromatography BSA: Bovine serum albumin RPMI: Roswell park memorial institute medium DAPI: 4,6-Diamidino-2-phenylindole DMSO: Dimethyl sulfoxide g/mL: Micrograms per milliliter mV: Millivolt nm: Nanometer References Tarver, T

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