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Serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) peaked at 12 h in both mice genotypes (Figures 2a and b)

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However, when the researchers adjusted for other things , including siblings with autism, the increased risk was no longer statistically significant .[ref][ref] (Note that other researchers point out bias in that paper.[ref]) Another large study published in 2021 involving 73,000 children and mothers found that there was a 19% increase in relative risk of ASD with prenatal acetaminophen exposure.[ref] A 2025 meta-analysis of studies on acetaminophen use during pregnancy and infancy shows that the majority of studies and the higher-quality studies show a positive association between acetaminophen use and ADHD, ASD, or neurodevelopmental disorders.[ref] A couple of things to consider here