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in the mid-1930s [26], (b) produced mainly by fibroblasts [27], (c) to date consists of 28 types, numbered from IXXVIII, constituting about 8090% (type I) and 1015% (type III) in human skin [27], (d) provides tensile strength and elasticity to withstand mechanical forces in everyday activities [26,27,28,29], and (e) mainly composed of the amino acids glycine (Gly), proline (Pro), and hydroxyproline (Hyp), along with its typical triple-helix structure [26, 27, 29]

While the prevailing view has shifted toward the belief that tinnitus originates within the central auditory system (CAS), peripheral auditory damage has consistently been identified as the primary risk factor 4,5,6,7,8

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MS2 spectra were fragmented by higher energy collision dissociation (HCD) (normalised collision energy (NCE) = 30) activation mode, and the ion-trap was selected as the mass analyser