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See Table 3 for class specific item response probabilities in the 4-class model, depicted in Figure 1 as a radar plot 28

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The treated samples were added to black 96-well plates and mixed with working reagent (5 mL: 4.82 mL 1x buffer, 75 L Reagent A, 50 L HRP, 50 L cholesterol oxidase, 5 L cholesterol esterase)