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Hepatic F4/80 + cells were isolated from dissociated liver samples with Anti-F4/80 MicroBeads UltraPure (mouse, Miltenyi Biotec) as per manufacturers protocol and flash-frozen in liquid nitrogen before being stored at 80C for mRNA extraction

After breastfeeding, or once cleared by a healthcare professional

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The results were striking: participants receiving the highest 12 mg dose achieved mean weight loss of approximately 24.2% of baseline body weight at week 48, compared to just 2.1% with placebo - representing an astounding 22 percentage point placebo-adjusted treatment effect