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Here is the complete chain of calculations: Step 1: Calculate concentration Concentration = Peptide amount (mg) / Water added (mL) Step 2: Convert your desired dose to mg (if given in mcg) Dose in mg = Dose in mcg / 1000 Step 3: Calculate the volume needed Volume (mL) = Dose (mg) / Concentration (mg/mL) Step 4: Convert volume to syringe units Units = Volume (mL) x 100 (for a 100-unit/1 mL insulin syringe) Worked example: BPC-157 Say you have a 5 mg vial of BPC-157

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Cells were passaged at confluence with 0.05% trypsin and maintained in a 37 C incubator with humidified atmosphere of 5% CO 2 and 95% air