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(1): Assessment of vesicular size, polydispersity index (PDI), and Zeta-potential measurements A dynamic light scattering approach was utilized to measure vesicle size, PDI, and zeta potential of the developed LS-loaded nanospanlastics by Zetasizer device (Malvern ZS, Ltd., Malvern, UK)

Gene expression analysis Total RNA was extracted from cells or tissues using Trizol (Thermo Fisher Scientific) or the RNeasy kit (QIAGEN)

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