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Immunocytochemistry Immunolabeling of NPCs and derived neurons was accomplished by utilization of a standard two-day protocol with: 15 min fixation in 4% PFA, 10 min permeabilization in 0.1% triton-PBS and 30 min blocking with 5% donkey serum

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Kristin has served as an Aesthetic Physician Assistant for the past 17 years working in Beverly Hills, CA, Calabasas, CA and now in Nashville since her move here in early 2019

The intestinal tissue was thoroughly cleaned with L-15 medium and digested with 0.4 mg ml 1 collagenase XI (Sigma, Poole, UK) in high-glucose DMEM at 37 o C, as described previously