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Bipolar disorder: A landmark RCT (n=75) found that NAC (1,000 mg twice daily for 24 weeks) produced a significant improvement in depression scores (Montgomery-Asberg Depression Rating Scale) in bipolar disorder as an adjunct to usual treatment (Berk et al., Biol Psychiatry, 2008) [74]

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To prepare the RNAi-expressing bacteria, single colonies were used to inoculate small cultures (15 mL) before being scaled up to larger cultures in terrific broth (TB) supplemented with 100 g/mL carbenicillin