Organs were homogenized either by repetitively rolling a serological pipette over the samples in sample bags (liver) or by bead beating using a Precellys 24 Touch homogenizer (Bertin Technologies) at 5,000 r.p.m
Proteins were then separated via 8% SDS-PAGE (Servicebio, Wuhan, China), transferred to PVDF membranes, and blots were then blocked for 2 h at room temperature with 5% non-fat milk prior to incubation overnight with appropriate primary antibodies at 4C
In case by accident, this medicine goes into your ears, nose or mouth, immediately rinse it with water
Patients with acute injuries, severe pain, loss of function, swelling, neurological symptoms, or other concerning changes should seek appropriate in-person medical evaluation