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After removing half of the culture medium, neurons at 6070% confluency were transfected by adding 100 l transfection mix per 1.76 cm 2 well dropwise onto the cells

An update for this topic was in progress during this manuscripts development

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Modified fasting therapy is defined as a form of very-low-calorie-diet (VLCDs), which has been applied in an integrative medicine ward in Germany for decades [4]