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Definitive diagnosis can be confirmed with skin biopsy that typically shows any of the following: Evidence of neutrophilic infiltration within and around the vessel wall with the signs of clear dust or leukocytoclasia (disintegration of neutrophil nuclei into fragments) Fibrinoid necrosis or fibrin deposition within and around the vessel wall Signs of damage to the vessel wall and surrounding tissue such as damaged endothelial cells or extravasated red blood cells

Consistent frequency is paramount for sustained signaling and optimal results

The samples were pooled and sequenced with a NextSeq 500 sequencing machine (Illumina) to give 61-bp paired-end reads