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Measurement: Measure the optical density (OD) at 450 nm using a microplate reader immediately (within 15 minutes of adding the stop solution)

Skipping the temperature equilibration step: Cold-to-warm condensation can introduce moisture before reconstitution begins

In 3-6 hpi ring-stage resistant parasites, we demonstrated lower levels of hemoglobin-derived heme, which is the crucial activator of artemisinin in parasites

Because rifaximin is minimally absorbed, it is concentrated in the gastrointestinal tract, which in turn alters gut microbiota composition and function, affects bile acid levels and composition, and exerts anti-inflammatory action and alters neurotoxin levels, all of which are implicated in the pathogenesis of cirrhosis complications