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It is likely that some steps of the biotransformation of arsenic that involve the formation of glutathione-bound species can occur also involving individual cysteine thiol groups or pairs of vicinal thiol groups in proteins, such as the catalytically essential thiol groups of enzymes and the regulatory elements of effector proteins that can be modulated by reversible glutathionylation (see further)

Both concentrations extended the lifespan of the worms, with the 5 mM caffeine treatment showing a more pronounced effect, increasing the lifespan of the N2 strain by 22.22%