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The erythrocytes were then lysed with lysis buffer (BD Pharm Lyse, BD Bioscience, San Jose, USA) for 10 min followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and resuspension of the cells in Hepes-buffered saline (HBS, 14 mM Hepes, 0.9% NaCl)

The time for normal skin to scab after transplantation has been reduced from 10-14 days to 5 days.

Evidence suggests oral tetracyclines (ie, doxycycline) and macrolides (ie, erythromycin) for one-to-three months improves tear film stability and increases meibomian secretions where ocular rosacea is severe